Naturally occurring microorganisms are having ability to produce the various enzymes and compounds. Indeed, it is boon of god because nowadays most of these compounds are industrially important and human welfare. Biosurfactant is one of the important compounds involved in bioremediation, which can be produced by various microorganisms. Many species of microorganisms are known to produce biosurfactant by the by the utilization of hydrocarbon. The biosurfactant producing bacterial strain isolated from petrol bunk sample (ADYAR, Chennai). The isolated bacterial strain was identified as Bacillus licheniformis. In the production optimization studies, the bacterial strain needs temperature around 37°C,PH 7, glycerol as carbon source, incubation period of 36 hrs and initial glucose concentration of 3mM as optimum parameter for better production of biosurfactant. The biosurfactant produced by above organism was acid precipitated and extracted as partially purified one using ethyl acetate extraction technique. The extracted partially purified biosurfactant characterized through TLC and the presence of corresponding amino-acid (Glu, Asp, Val, Leu, Ile) was detected whose having Rf value of 0.83.When studied with application, biosurfactant inhibited the growth of Bacillus subtilis thus forming clear zone of lysis around the well. Moreover it seemed to having antibacterial effect against 5 gram positive bacteria and no effect against 2 gram negative bacteria tested. The systematic position of Bacillus licheniformis BIOS PTK1 was determined based on 16S rRNA sequence. BLAST homology analysis revealed that the sequence of Bacillus licheniformis BIOS PTK1 showed 100% sequence identity with more than 60 Bacillus licheniformis.
