Background: Stress in work can be prevented from occurring and can be faced without getting a negative impact. Stress management is more than just overcoming it, namely learning to overcome it adaplively and effectively. A person experiencing stress or being in a stressful condition will affect the quality of his work such as a nurse, it will affect the quality of nursing services because it is not concentrated in providing services. The purpose of this study was to determine the relationship between the work stress factors of nurses and the quality of nursing services in the Hospital Emergency Room. Method: the research design used was descriptive analytic with a cross sectional approach, the number of respondents was 49 people, determining the size of the sample using accidental sampling. Data collection using a questionnaire. Data processing using SPSS 21.0 computer program which is presented in the form of tables, diagrams and narratives. The analysis carried out is univariate and bivariate with a significance level of α 5 0.05. Results: from the results of the data obtained there is no relationship between education, work environment conditions with the quality of nursing services in the Hospital Emergency Room Installation with p> 0.05, and there is a relationship between workload and the quality of nursing services in the Home Emergency Room Installation Pain with p = 0.003. There is a relationship between the factors of interpersonal relationships with the quality of nursing services in the Hospital Emergency Room at p = 0.004. Conclusion: based on the results of this study that the work stress factors of nurses with the quality of nursing services in the Hospital Emergency Room Installation are workload factors and interpersonal relationships.