Introduction: Hepatitis B poses a major health concern and is the world’s most common blood borne viral infection placing dental professionals at higher occupational risk. Materials and Methods: The present study was conducted to assess knowledge, attitude and practice among 97 dental interns of Sinhgad dental college, Pune. A cross sectional study was conducted. A predesigned questionnaire was the tool of data collection. Data analysis was in the form of frequency and percentage and chi-square test were done. Result: The present study showed that, only 19.6% and 48.5% of them know the modes of transmission and diagnostic tests respectively. Majority of them had a positive attitude on HBV. 78.4% considered vaccination of HCW very important and 55.7% considered vaccination as precautionary method but only 76.3% of them are vaccinated as per the WHO regimen. Conclusion: Hence, there is an imperative need for health education to improve the knowledge & practice of interns towards HBV
