In vitro shoot multiplication and micropropagation have been carried out from the shoot tip of Digera muricata (L.) Mart., a member of Amaranthaceae. The shoot tips were cultured on MS medium supplemented with different concentrations of BAP and Kin ranging from 5 to 25 µM alone or in combination of both. Among the two cytokinins tested, 10 µM BAP was found to be the best for shoot proliferation. However, combination of Kin with 10 µM BAP further enhanced the rate of shoot multiplication. An average number of 16.8 shoots per explant with 100% shoot induction frequency were observed on MS medium containing 10 µM BAP and 2 µM Kin The regenerated shoots were cultured on half strength MS medium containing different concentration of NAA and IBA for root induction. Among these two auxins, 9 µM NAA was found to be the best hormone for rooting. The well rooted plantlets were hardened.
