A inexpensively low-cost Nano hydroxyapatite (Ca10 (PO4 )6 (OH)2 was synthesized from the biomaterial such as land snail-shell (Helix Pomatia) using di-ammonium hydrogen phosphate (NH4 )2HPO4 under microwave irradiation. The grained powder of land–snail shell was dissolved in diluted hydrochloric acid and a solution di-ammonium hydrogen phosphate was slowly added to the mixture while maintaining the pH at 9-10 using ammonium hydroxide(NH4OH), followed by microwave irradiation for 30 min .The Nano hydroxyaptite (nHAP) after microwave irradiation was collected and calcined at 900° C for 2h, The synthesized HAp as nano crystal of 20-30 nm scale was soaked in simulated body fluid (SBF) for various period of time likely 7, 14 and 30 days respectively. After the particular period of soaking; the residue was collected, washed and dried at hot air oven. The powdered mass was characterized by XRD, FTIR, FE-SEM, and FE-TEM analysis. The prepared powder sample using land snail shell under microwave irradiation is hopeful source of carbonated hydroxyapatite (CHAP) with tremendous properties and can be considered for a better bone substitute material and the prepared HAP powder due to the formation of apatite on its surface was observed.
