Mosquito vectors are spread deadly diseases such as malaria, dengue, chikungunya and lymphatic filariasis now a day nanotechnology used for eco-friendly approach to control of mosquitoes and platforms in the area microbial biology have attracted remarkable attention. This research proposed a novel method of biosynthesis silver nanoparticles using seaweed, Turbinaria ornata. Nanoparticles were characterized by UV–vis spectroscopy, FTIR, SEM, EDX and XRD. In laboratory assays, the T. ornata-synthesized silver nanoparticles against A. stephensi the LC50 values ranging from 9.150 ppm (I) to 24.977 ppm (pupae); LC50 values for Cx. quinquefasciatus ranging from 11.963 ppm (I) to 27.643 ppm (pupae) and LC50 values for A. aegypi ranging from 10.214 ppm (I) to 23.630 ppm (pupae). In addition, the antibacterial activity of T. ornata -synthesized AgNp was evoked inhibition zone wider. Overall results of this study demonstrate that the green synthesized nanoparticles potential to be used as a perfect eco-friendly method for the control of mosquitoes and microbes
