One hundred and twenty skin swabs specimens were collected from acne patients. Thirty eight (45.2%) specimens were identified as Staphylococcus aureus and twenty seven (32.1%) specimens were identified as Propionibacterium acnes. Most of S. aureus isolates were susceptible to methicillin and all isolates were susceptible to vancomycin. All P. acnes isolates were susceptible to all antibiotics used in this study. DNA was extracted and purified from the most sensitive isolates (P. acnes P7 &S. aureusS24). The effects of S. aureusw hole cells were higher than the effects of P. acnes whole cells, while both isolated DNA were able to produce more severe inflammatory changes than the whole bacterial cells. Furthermore, increasing the DNA concentration caused an increase in the intensity of skin inflammation. Moreover, the effects ofhigh GC ratio P. acnes DNA developed the highest inflammatory effect than the low GC ratio S. aureusDNA
