a new rp-hplc method for simultaneous estimation of telmisartan and cilostazol in synthetic mixture

Research Article
Riddhi R. Patel and Dilip G. Maheshwari
RP-HPLC, Telmisartan, Cilostazol, Simultaneous estimation.

A simple, sensitive, precise and accurate RP-HPLC method has been developed for simultaneous estimation of Telmisartan and Cilostazol in the synthetic mixture by gradient elution. Chromatographic elution has been carried out on an Enable C18G (250 × 4.6 mm, 5 µm) column by using the mobile phase Potassium dihydrogen phosphate buffer (10mM): Methanol: Acetonitrile (30:10:60 v/v/v, pH 5.8). The flow rate was 1.0 ml/min. Detection was monitored at 257 nm using UV detector. The retention times of Cilostazol and Telmisartan was found to be 5.49±0.1min and 9.62±0.1 min respectively. The linearity was observed in the concentration range of 2-10 µg/ml for the Telmisartan and 4-20 µg/ml for the Cilostazol. The methods were validated and shown linear response for Telmisartan and Cilostazol. The limit of detection and limit of quantification for Telmisartan was found to be 0.041 and 0.125 µg/ml, respectively and for Cilostazol was found to be 0.038 and 0.117 µg/ml, respectively. The precision (intra-day, interday, repeatability) of methods were found within limits (RSD <2%). Accuracy was determined by recovery studies. Validation of proposed methods was carried out according to ICH guidelines.