The present research based on the phytochemical analysis and antimicrobial activities of Merremia dissecta (Jacq.). It is a member of family Convolvulaceae. The shade dried leaves powder was extracted with different solvent such as petroleum ether, benzene, chloroform, acetone and ethanol. The phytochemical evaluation revealed that the leaves contain alkaloid, glycoside, saponins, carbohydrates, tannin and flavonoids. These plant extract of Merremia dissecta were examined using agar disk diffusion method against Gram positive and Gram negative bacteria. Antimicrobial activity tested against one Gram positive (Bacillus subtilis) and four Gram negative (Salmonella typhi, Proteus vulgaris, Enterobacter aerogenes and Proteus mirabilis) pathogenic bacteria. B. subtilis showed highest (17.0 mm) zone of inhibition with ethanol extract of leaf.
