Aim: The aim of this study was the molecular detection of multi-drug resistant dental pathogens in association with the infection of root canal system.
Methods and Material: Study was conducted during the period from March 2013 to September 2013. Totally 94 samples were collected aseptically and the yielded isolates were biochemically characterised and screened through antibiogram study. Standard disc diffusion method was used for antibiotic sensitivity study of Staphylococcal isolates. 16S rDNA sequence was analyzed for the confirmation of multidrug-resistant isolates.
Results: The isolates Enterococcus sp., Staphylococcus sp., Bacillus sp., Streptococcus sp. and Candida sp. were found and reported more in females (n=55) than males (n=39). Among the 19 Staphylococcus isolates 41.7% were S.sciuri, which show a higher percentage of antibiotic resistant (21.05%). In root canal system, this is the first report of isolation of multidrug-resistant S.sciuri. 16S rDNA sequencing results confirmed the multidrug resistant Staphylococcus strains and were 99% similar to S.sciuri.
Conclusions: Study showed the prevalence of S.sciuri in root canal system. The level of antibiotic susceptibility of the S.sciuri strains clearly indicated the emergence of multidrug-resistant pathogens. This information alarms the clinicians to concentrate on MDR pathogen while treating root canal infection.
