In the recent research investigation suggested that the population of fungi from Pudukkottai district and screening of pectinase enzyme from soil fungi were performed. The population dynamics of soil fungi such as Alternaria sp, Aspergillus awamori, A.flavus, A.fumigatus, A.nidulans, A.niger, A.oryzae, A.terreus, Chaetomium sp, Fusarium solani, Fusarium sp, Penicillium citrinum, P.chrysogenum and Trichoderma viride was isolated and identified from Pudukkottai town bustand and Malaiyur soil. The fungi identified by using standard manual. On the other hand the studies on the primary screening of pectinase enzyme from soil fungi were determined. The screening of pectinse enzymes with the zone of measurement was 15, 12, 10, 10, 15, 15, 20, 10, 15, 17, 10, 14, 15, 5 and 20 mm with respective fungi were analyzed. According to the screening of fungi for enzyme production ratio was increased. The maximum zone of measurement was 45 mm with Aspergillus niger recorded and minimum zone of measurement was 12 mm recorded respectively. Alternatively different substrate materials are produced by agricultural waste and fruit processing industry which possess considerable disposal problem and ultimately leads to pollution.
