At present, silver nanoparticles have found a distinctive place in the field of biomedicine due to their unique antibacterial property. In the present study, chitosan a non toxic bioploymer extracted from Portunus pelagicus crab shell wastes was used as a stabilizer and a reducing agent in the biosynthesis of silver nanoparticles. Chitosan mediated silver nanoparticles was synthesized and the antibacterial efficacy of both has been examined. Crab shell wastes were demineralized and deproteinized to yield chitin and it was partially deacetylated to yield chitosan. Antibacterial activity was conducted against two gram negative E.coli, Pseudomonas aeruginosa and two gram positive Bacillus subtilis, Staphylococcus aureus bacterial strains using disc diffusion assay. Streptomycin was used as positive control and acetic acid was used as negative control. Antibacterial activity of chitosan mediated silver nanoparticles showed pronounced activity compared to chitosan. Thus it can widely be used as good antimicrobial agent in biomedical and pharmaceutical industries.
