The objective of the present study is to assess the microbial contamination in water, sediment and fish of lotic water body, Ambrampalayamriveras it is observed a direct and indirect disposal of wastewater, agricultural drainage and domestic wastes into the water body. Samples to be analysed were collected from the sites exposed to incidental waste disposals. The core composition of bacterial communities consisted of Aspergillusfusarium, Aspergillusniger, Aspergillusflavus, Enterobacter cloacae, Bacillus subtilis, Shigellasp, Escheria coli, Vibrio cholera, Pseudomonas sp, Aeromonashydrophila, Aspergillusflavus, Cladosporium and Rhizopus were identified in water, sediment and fish samples of Ambarapalayam river. Minimum count of Aspergillusflavus, Cladosporium and Rhizopus are recorded in the water samples. Rhizopuswas recorded minimum level in the analysed fish sample. Enterobacter cloacae, Bacillus subtilis, Shigellasp, Vibrio cholera and Pseudomonas sp. are recorded minimum in study site during the study period in the analysed sediment samples. Among the microbial community estimated the higher recorded was total faecal coliform count 172×109 (MPN/g); 150×109 (MPN/g);128×109 (MPN/g) in fish, water and sediment respectively. The site selected for analysis were observed to be contaminated with high rate of total faecal coliform and total fungal count in fishand water followed by sediment which is an indicator of to spot getting spilled with untreated pollutant both directly and indirectly. Thus the present study confirms that the prime lotic water body of PollachiTaluk, Ambarampalayam village is slowly getting degraded due to high range of pollutant mixing into it which in turn will bring ill effects to public inhabiting in and around to the people who use this water and fish for consumption.
