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Industrial wastewater and soil sample is one of the most essential source of contamination in the surface environment. Fungi provide much valuable service to the mankind and in the soil ecosystem and also the causes of different types of disease. In addition, to produce chemical with different odours and tastes in water. The continuous irrigation of wastewater results dramatic change in the soil nutritional status may favor certain fungal groups while hampering the growth and diversity of spores. In the present study that the isolation of fungi from 10-3 dilution factors were in the highly population diversity from the high energy battery effluents waste water percolated soil sample of Avoor, Tiruchirappalli. The result obtained showed that the most widely distributed fungi of Alternaria alternata, Aspergillus flavus, Aspergillus fumigatus, A.vercicolur, Cladosporium sp, Cruvalria lunata, Fusarium sp, F.oxysporum, F.solani, Rhizopus sp, R.oryzae, Trichoderma viride and Verticillium sp. grew well in soil containing heavy metal. The experimental results indicated that, most of the physiochemical properties such as pH, electrical conductivity, organic carbon, organic matter, available N2 , Phosphorus, potassium, iron, manganese, calcium, magnesium and sodium were analyzed. This heavy metals also investigated such as As, Cd, Ni, Pb, Zn, Cr and Cu from battery effluents contaminated soil. Furthermore, the results indicated that fungi of metal contaminated soil have high level of metal tolerance and bio sorption properties from the fungi were analyzed.