Every year large number of people suffers diabetes which may also be fatal. Approaches have been made from time to time which included in vivo models but have certain limitations so it becomes the need of the hour to develop in vitro methods. In-vitro methods here comes into play with which attempts have been made to establish beta cell lines that can retain normal regulation of insulin secretion but have mostly been unsuccessful. The availability of such cell lines is limited and hence researchers have used X-rays and viruses to induce insulinomas, derivation of cells from transgenic mice or even from non-islet cells to produce immortalized cell cultures. In this article, we have briefly discuss in-vitro models over-viewing cell line techniques for producing beta cells from animal and human origin by throwing the light upon the creation of continuous cell line from beta cell of pancreas by expression of SV40 antigen which has the capability of producing transformed beta cell lines with improved response to glucose by expressing various enzymes like GLUT and glucokinase. The need is to produce a normal cell line of human origin.
