optimization of cellulase and bioethanol production using saw dust as substrate by bacillus subtilis mtcc 121

Present study was aimed to determine the optimum conditions (Concentration, pH, Temperature and Inoculum size) of Bacillus subtilis MTCC 121for cellulase enzyme production and to examine the effect of inorganic and organic nitrogen sources and surfactants on the saccharification process from saw dust. The highest cellulase activity was observed at 4% concentration of pretreated saw dust (lignocellulosic biomass). Optimum pH and temperature of the medium for cellulase production by B. subtilis MTCC 121 were 8.0 and 400C. NH4H2PO4 and Urea were found to be best nitrogen sources for bioethanol production where as surfactants showed negative impact on saccharification process.