Optimization Of Lignolytic Enzymes Produced By Aspergillus Strains In Anthraquinone Dye Degradation

Research Article
Srinu A., Lenin Kumar B., Siva Prasad B.V., Murali S., Vijaya Lakshmi D and Prasad DVR
DOI: 
http://dx.doi.org/10.24327/ijrsr.2018.0902.1538
Subject: 
science
KeyWords: 
Isolation, Fungal strains, Decolorization, Lignolytic enzymes, Optimization.
Abstract: 

The present work mainly focused on the decolorization studies of anthraquinone dyes (Remazol brilliant blue-R (RBBR), Brilliant blue-R (BBR) and Acid blue-25 (AB-25)) using different fungal strains (Aspergillus flavus strain AJ, Aspergillus terreus) isolated from industrial effluents. The maximum decolorization activity was recorded with Aspergillus flavus strain AJ (AB-25-83.0%) and less activity showed by Aspergillus terreus (BBR-46.2%). These two fungal strains also tested for production of their lignolytic enzyme activity i.e Laccase, Manganese peroxidase (MnP) and Lignin Peroxidase (LiP) activity at 30o C. Among all tested MnP (196.5U/ml) enzyme produced maximum by Aspergillus flavus strain AJ and minimum LiP (12.9 U/ml) enzyme produced by Aspergillus terreus. Further these enzymes were also optimized with various parameters like temperature, pH, Carbon & Nitrogen sources.