Optimization Of Pigment Production Using Placket Burman And Surface Response Methodology

Research Article
Naziya M.A.Rehman and Prashant P. Dixit
DOI: 
http://dx.doi.org/10.24327/ijrsr.2017.0807.0516
Subject: 
science
KeyWords: 
Pigment production, Placket Burman, Box Behenken
Abstract: 

Research on microbial pigments has been an important and interesting field, as microbial pigments have medicinal, nutritive and other beneficial characters. Many microbial pigments have shown good antibacterial, antifungal, antioxidant, antitumor, anticancer activities. Microbial pigments are considered as good alternatives to the artificial colorants, as many microbes produce intense pigments. Artificial colorants are harmful for human, animal and environmental health. Microbial pigments might be involved in many processes which are not known till now. They might act as drug or might possess some unique pharmacological properties. Many reports tell us that there is a great effect of environmental factors and media components on microbial pigment production, thus there is a need to optimize the media components for microbial pigment production, if we want to produce them at large industrial level. Present research study was aimed to optimize the media components for the maximum pigment production. A Placket Burman design was used to screen main media variables which have a great influence on pigment production. The significant variables were optimized by using response surface Box Behenken methodology. Peptone, soybean meal and pH were screened as main significant factors by these optimization methodology.1% peptone, 1%soyabean meal and pH 7 was found as optimum for increased pigment production.