optimization of process parameters for the enhanced production of α -amylase using a.niger in submerged fermentation

Optimization of five process parameters - pH, temperature, fermentation time, inoculum size and substrate concentration - were attempted for the optimal production of alpha amylase using central composite design of response surface methodology by A.niger MTCC-282 under submerged culture conditions. The optimization process was carried out using the central composite design and response surface methodology. Maximum amylase activity of 25.0 U/ml was obtained at the fermentation time of 75.55 hr when an inoculum size and substrate concentration of 5.17 % and 21.2 g/L respectively were used at a pH of 4.84 and temperature of 29.93 oC.