Bromelain is a mixture of enzymes found in pineapples (Ananas comosus) that digests proteins (proteolytic). The active factors involved are biochemically characterized only in part. The mixture of the enzyme can be purified using various techniques such as Precipitation, ATPS (Aqueous TwoPhase System), RMS (Reverse Micellar System), IEX (Ion Exchange Chromatography) etc. The current review discusses methods in relation with enzyme recovery and specific activity of Bromelain enzyme. The implications will be helpful to researchers for selection of suitable purification methods. These methods should be precise, cost effective and reproducible. Based on the said criteria Ammonium sulphate precipitation followed by Dialysis and subsequently by IEX were observed to have minimum loss of activity and higher yields of product were obtained. Bromelain has earned growing acceptance and compliance among patients as a phyto-therapeutical drug due to its efficacy after oral administration, its safety and lack of undesired side effects. This review will help to strategize the purification process for Bromelain and thus use it for various applications.
