Nature represents a formidable pool of bioactive compounds and is more than ever a strategic source for new and successful commercial products. The potential of using microbes as biotechnological sources of industrially important enzymes has triggered renewed cynosure in the exploitation of microbial communities as bioreactors. Among these bioreactors, amylases have received great deal of attention. In these endeavours this study is conducted to search for new amylase with waste pod biomass of Pithecellobium dulce as substrate. A partial purification and characterization of the amylase from Aspergillus flavus was carried out in this study. The extracellular amylase extract was concentrated using ammonium sulfate precipitation and dialysis. The optimum conditions for the partially purified amylase were evaluated. The optimum pH and temperature were observed 6 and 60°C respectively. Furthermore, the analysis of kinetic showed that the enzyme has of 1.43 mg/ml and max of 250 mol/min/mg. The results indicate that the enzyme reflects their potentiality towards industrial applications.
