The present study was aimed to investigate the qualitative and quantitative analysis of the major bioactive constituents of medicinally important plant Manilkara hexandra (Roxb.) in its aqueous extract of leaves of the plant. Studies were carried out in terms of aqueous extraction, total extractive values, qualitative and quantitative estimation of phytochemicals. The percentage value of yield extraction was found to be 5.15%. The preliminary phytochemical analysis confirmed the presence of phenols, flavonoids, saponins, carbohydrates, alkaloids and glycosides. The total phenolic content ranged from 31.857 mg/100mg of dry weight of extract, expressed as gallic acid equivalents. The total flavonoid concentrations varied from 25.097 mg/100mg, expressed as quercetin equivalents. It signifies that results revealed the presence of various bioactive compounds which could be further exploited and isolated for their potential applications for medicinal purposes
