The present investigation was carried out to screen the phytochemical and antimicrobial properties of Cheilanthes bicolor collected from Agumbe area, Karnataka, India. The dried whole plant material was extracted by soxhlet method using acetone, chloroform, methanol, ethanol and aqueous solvent systems. The phytochemical screening for the presence of carbohydrates, proteins, phenols and tannins were carried out with standard protocols. Antimicrobial activity of plant was carried out with 50, 100, 200, 500 and 1000 μg/ml concentration against four bacterial strains i.e., Salmonella typhii, Klebsiella pneumoniae, Staphylococcus aureus and Escherichia coli with Streptomycin as positive and respective solvents as negative controls. Antifungal activity was carried out against Aspergillus niger and Sclerotium rolfsii. Among different solvent systems used aqueous yielded good quantity of extract followed by methanol and acetone. The phytochemical study showed the presence of biomolecules such as carbohydrate, protein, tannin and phenols. The chloroform extract exhibited better antimicrobial activity against Escherichia coli and Salmonella typhii and the ethanolic extract showed better activity against K. pneumoniae and S. aureus. Ethanolic extract at 50μg, 500μg, and 1000μg exhibited antifungal activity against A.niger and acetone extract at 50μg showed activity against A.niger
