Introduction: Piper betle (L) is a common plant cultivated in Asian countries, leaves of which are used in folk medicine for the treatment of various disorders. Aim: To evaluate the anti-bacterial efficacy of 4% Piper betle (L) leaves extract mouth rinse on the Human Gingiva. Materials and Methods:45 systemically healthy patients,15 years and above with mild to moderate Gingivitis were randomly divided into: Group A: Distilled Water (Placebo). Group B: Commercially available 0.2% Chlorhexidine gluconate (Positive Control). Group C: 4% Piper betle leaves extract mouth rinse (Test Group). The supra-gingival plaque samples were collected at baseline and 15 days and Colony Forming Units (CFU) were recorded. Plaque Index(PLI), Gingival Index (GI) and Sulcus Bleeding Index(SBI) were recorded at baseline,7th day and 15th day, thorough scaling was done at baseline, then provided with the mouthrinse depending on which group they belonged .Statistical analysis was done using Kruskal-Wallis, Mann-Whitney and the Wilcoxon matched tests. Results: Test group showed significant reduction in PLI, GI, SBI at 7th and 15th day compared to baseline values. Conclusion: The present study demonstrated the anti-inflammatory efficacy with 4% aqueous extract of Piper betle linn.
