Proniosomes are one of the important novel drug delivery carriers of various drug molecules. Aceclofenac potent analgesic anti inflamatory agent used for the treatment of inflammation. The main objective of the study was to develop proniosomal containing aceclofenac for transdermal delivery using different ratios of cholesterol and non-ionic surfactants in order to achieve a sustained release of drug on topical administration. Proniosomes were prepared by using slurry method and evaluated for angle of repose, entrapment efficiency, thickness, folding endurance, percent moisture loss and absorption, drug content and in-vitro diffusion studies. As the concentration of the cholesterol decreases the entrapment efficiency decreases due to the low vesicle size formation the in-vitro diffusion and kinetic analysis were done for proniosomal patches. It showed the release of 51.28% at 8th hr and fitted into Zero order and follows non[1]fickian diffusion mechanism. The best formulation (F6) was composed of cholesterol and surfactant in the ratio of 1:2 showed the better sustain action. From the study it was observed that proniosomal transdermal patches are very stable and promising sustained delivery system for Aceclofenac
