The purpose of this study was to determine the prevalence and antibiogram profile of Yersinia enterocolitica and to assess the carrier status for Y. enterocolitica in milking cows of Tirupathi region, Andhra Pradesh, South India. Altogether 120 samples were processed for isolation of Y. enterocolitica in which 50 samples were raw milk and 50 samples were faecal samples of lactating dairy cows. The samples were collected from different farms located in Tirupathi region, under aseptic conditions. All the samples were processed for isolation and identification of Y. enterocolitica as per standard protocol. Evaluation of antibiotic sensitivity pattern of Y. enterocolitica was assessed by Kirby-Bauer method. The overall prevalence of Y. enterocolitica was observed in 25% of the collected samples which comprising of cow milk (10%) and lactating dairy cow fecal samples (35.71%) based on colony characters and biochemical reactions. All the Y. enterocolitica isolates were resistant to more than one antibiotic and no isolates were susceptible to all the antibiotics. Y. enterocolitica isolates were highly resistant to Streptomycin and Penicillin (96.67%). Least resistant against Amoxycillin (6.67%) and none was resistant to Co[1]trimaxazole. The predominant antimicrobial resistance pattern were Streptomycin – Penicillin - Oxacillin with 66.67% of isolates shown multiple antibiotic resistance indices of more than 0.2.
