The study was initiated with the objective to identify the role of G6PD deficiency in chronic kidney disease patients with unknown etiology (CKD-U) in North Central Region (NCR) of Sri Lanka. 104 recently diagnosed, biopsy proven CKD-U patients (cases) and 208 age and sex matched controls were selected from same areas. Demographic information were collected and G6PD activities were determined in both cases and controls. The severity of G6PD deficiency was interpreted. Collected information were analyzed by using two-way ANOVA experimental design model and mean separation. The mean G6PD activity of the case group was significantly lower (p <0.001) than the control group. Twenty percent (20%) of the patients had G6PD deficiency whereas only 2% in control group. Smoking, histories of malaria, alcohol consumption were significantly contributed for the disease. Prevalence of G6PD deficiency was high among CKD-U patients and which may play a major role in the pathogenesis of disease
