Background: Wound infections continue to be problematic in clinical practice where empiric treatment of infections is routine. These infections play an important role in development of chronicity consequently delaying wound healing. Objectives: For identification of bacterial pathogens present in infected wounds and for determination of their antimicrobial susceptibility pattern from patients with pus and/or wound discharge. Methods: A prospective study was conducted at Burdwan Medical College and Hospital over a period of three months (from March to May, 2017). Wound swab samples were collected from each study participant and inoculated onto appropriate media. The bacterial pathogens were identified using standard microbiological methods, Vitek 2 (bioMérieux) GN-ID card and 16S rRNA gene sequencing carried out by ABI 3500 Genetic Analyzer. Antimicrobial susceptibility tests were performed using disk diffusion technique following Kirby-Bauer method. Methicillin resistance in staphylococci was determined by cefoxitin disk diffusion (DD) test. ESBL was detected by phenotypic confirmatory disk diffusion test (PCDDT) using ceftazidime alone and in combination with clavulanic acid. MBL detection was done by imipenem-EDTA combined disk diffusion test (CDDT). Results: Out of total 153 specimens 114 bacterial isolates (38.6% Gram positive isolates and 61.4% Gram negative isolates) were recovered showing an isolation rate of 74.5%. Klebsiella pneumoniae were the most frequently isolated bacteria accounting for 27(23.7%). Polymicrobial infection was found in 8(7%) of the infected wounds and was mainly constituted by two species. Moreover, 8 MRSA isolates and 3 ESBL and MBL producing Enterobacteriaceae isolates were obtained. 52.6% of bacterial isolates had MAR index > 0.2.Out of them 38(63.3%) were MDR isolates. Conclusions: Prevalence of bacterial infections reached in high amount showing Klebsiella pneumoniae the most dominant one. As multidrug resistance among bacterial population is a major threat in recent years, so a constant and careful worldwide surveillance for them is urgently warranted
