Chromobacterium violaceum a Gram-negative bacterium produces a spectacular pigment called violacein. Violacein is a quorum-sensing metabolite compound and is also an active antimicrobial, anticancer agent. Despite its efficiency as a potential drug, alone or in synergy with other active principles has not being completely demonstrated. With the advent of the multi-drug resistant uropathogenic strain E.coli, it becomes essential to find a new natural product(s) that could be effectively used as a therapeutic agent. This current research work is projected on the extraction of violacein from C. violaceum and its combinatory effect with commercial antibiotics against various pathogens. Violacein production was optimized and characterized by Gas -mass spectrometry and infrared spectroscopy. It was observed that violacein works synergistically with most commercial antibiotics and could be used as a drug in combination with other antimicrobial agents
