Regeneration competence of pseudobulb segments of Coelogyne ovalis, procured from in vitro grown cultures, was assessed in Mitra medium alone and in combinations with PGRs. Juvenility of the tissues, chemical stimulus, and position of the explants (apical/basal) were the key factors in initiating the response. The explants from pseudobulbs (>3cm long) did not regenerate whereas those from younger(<3cm in length) regenerated depending upon their position in the source organ..The response frequency and time taken for the development of plantlets also varied with the growth stimulus. Maximum number of shoots was obtained from the basal segments on medium supplemented with cytokinin to auxin in ratio of 10:5 in BM+BAP(10 mg l-1)+NAA(5 mgl-1 ) , 40 plantlets are formed after 14 wks..The apical segments formed shoots only from apical meristem. The shoots rooted in the same nutrient medium and formed .The regenerated plantlets were acclimatized & transfered to pots filled with moss, pinebark, brick and charcoal pieces mixture with 90% survival.
