Rehydration With Olive Oil Of Air Dried Buccal Smear: An Alternative To Routine Wet Fixation

Research Article
Sherin Singnarpi., Pratibha Ramani., Brundha M.P., Anuja N., Herald J Sherlin., Gheena., Abhilasha R., Giffrina Jayaraj., Don K.R and Arch
DOI: 
http://dx.doi.org/10.24327/ijrsr.2017.0804.0131
Subject: 
science
KeyWords: 
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Abstract: 

Aim: To evaluate the possibility of air dried smear and rehydration with olive oil instead of conventional method. Materials and Methods: 20 paired buccal smear taken from the healthy individual .one set labelled as wet fixation with 95% ethanol and other set labelled as dry fixation with rehydration with olive oil .they were graded blindly. Result: Statically significant similarities were seen between the two groups in terms of cellularity and staining intensity. Conclusion: The procedure of air drying and rehydration with olive oil is inexpensive, easy, convenient technique and almost equally effective to routine fixation.