Salinity Stress Induced Alternative Pathway Of Phosphate Dependent Glycolytic Enzymes In Anabaena Sp. Bhuar002

Research Article
Aparna Rai
DOI: 
http://dx.doi.org/10.24327/ijrsr.2018.0902.1673
Subject: 
science
KeyWords: 
Adaptation; Cyanobacteria; Glycolytic Pathway; Salt Stress
Abstract: 

Cyanobacterium Anabaena sp. BHUAR002 (Accession no. bankit1353506 HM235817) survive under energy depleted condition in salt stress because of different adaptations which facilitate organism survival. Inorganic phosphate which is the main source of energy in the form of ATP become diminished under stress condition, organism switch their dependency towards pyrophosphate which was unaffected under stress condition and all phosphate dependent glycolytic pathway enzymes alternate their pathway toward inorganic pyrophosphate dependent alternative glycolytic pathway enzymes., By observing enzyme activity under control and stressed condition, activity of main glycolytic pathway enzymes as inorganic phosphate or ATP dependent phosphofructokinase (PFP), Glyceraldehyde 3P-dehydrogenase, Phosphoglycerate Kinase and Pyruvate kinase found to be more in control. Whereas activity of alternative glycolytic pathway enzymes (inorganic pyrophosphate dependent enzymes) as PPi dependent phosphofructokinase (PFP), NADP- glyceraldehydes 3P-dehydrogenase, Phosphoenolpyruvate carboxylase (PEPC), Malate dehydrogenase and NAD – Malic enzyme was enhanced under stress condition.