Improving soil nutrients status by supplementing featured bacterial flora which can add and/or modify the complex/bound compounds into simpler form and those could be utilized further by plants has been attempted in present study. Phosphate is one of the key macronutrient which can act as a limiting factor for plant growth. Available P assists in deeper rooting and promotes plant development. Out of thirty three actinomycetes strains isolated from rhizosphere of different plants, twenty two were showing phosphate solubilization. All these strains solubilized phosphorus, expressed phosphatase .Quantitative phosphate solubilization was checked by Murphy and Riley’s method. When checked for the physiological parameters, P solubilization factor found to be best on 20th day of incubation with pH 7 at 28o C and recorded to be stable in all strains. In a pot assay, eight isolates showed to contribute in overall plant growth such as increase in root length and shoot length, few of them was even performed better than used positive control strain. In conclusion, candidate reported eight actinomycetes isolated from the rhizosphere of plants collected near Pune city found to posses important PGPR activity and strongly recommended as biofertilizer
