Plant growth promoting rhizobacteria (PGPR) are a group of beneficial microorganisms that colonize at plant rhizosphere, and induce plant growth by a wide variety of mechanisms. Over last couple of years, the use of PGPR is steadily increasing in modern agriculture as a possible alternative to replace chemical fertilizers, pesticides, and other supplements. In the present study, we have screened out a group of microorganism from the rhizospheric soil of Dhaincha (Sesbania bispinosa) on the basis of their PGPR characters. Twelve bacterial colonies with diverse growth features were isolated, and designated as DD1to DD12. They are characterized by their morphological, gram nature and biochemical properties. According to the efficiency finally three most efficient PGPR strains, i.e. - DD3, DD4 and DD6, were selected and further identified as Escherichia coli, Pseudomonas fluorescens, and Burkholderia sp. using 16 S rRNA sequencing technology. We believe using these PGPR strains individually or in combination as biofertilizer could be a viable step towards sustainable agriculture.
