Microbial populations are highly diversified in nature due to their metabolic diversity and genetic adaptability which enable them to survive in different ecological niches. The rhizosphere region of a specific plant inhabits specific microflora which produces numerous compounds involved in complex mechanisms such as biofertilization, phytostimulation and biocontrol activity. Among this, Pseudomonas genera plays a major role in biocontrol mechanisms due to their diverse metabolic compounds. These compounds are exploited as biocontrol agents against medically important vectors such as mosquitoes which pose a major threat to public health. Hence it is essential for identifying a potential candidate as alternative to chemical insecticides. In this context, the present study was conducted to isolate Pseudomonas fluorescens strains from different sources. Their toxigenicity were assayed and evaluated for biocontrol efficacy against medically important mosquito vector, Aedes aegypti (Linn).These effective strains proved to be potential candidate for bioinsecticide formulation and field application trails
