LEVELS OF AST AND ALT IN WISTAR RATS TREATED WITH HEAVY METALS RELEASED FROM ATAMONIA AND PEPSI CANS (=TINS=) AS COOKING POTS DURING FEEDING

Background: This study was done to estimate the serum levels of ALT and AST. Methods: Ninety adult males Wistar rats weighting about (160-220 gram each) were taken for the study. Serum ALT and AST levels were assessed by BioSystem, S.A, Costa Brava30, Barcelona. Spain2011). Results: On comparison of the controls and treated rats levels in serum samples, it was found that the difference was statistically significant. Conclusion: In the present study, Heavy metals from cooking pots made out of Atmonia & Pepsi cans such as Lead, copper, Aluminum, Ferrous and Arsenate give a significance increase (P≤ 0.01) in levels of ALT and AST.


INTRODUCTION
Heavy metals are natural constituents of the earth's crust, but in discriminate human activities have drastically altered their geochemical cycles and biochemical balance . This results in accumulation of metals in plant parts having secondary metabolites, which is responsible for a particular pharmacological activity, prolonged exposure to heavy metals such as cadmium, copper, lead, nickel, and zinc can cause deleterious health effects in humans, molecular understanding of plant metal accumulation has numerous biotechnological implications also, the long term effects of which might not be yet known (Yadav, 2010).  stated that if unrecognized or inappropriately treated, toxicity can result in significant illness and reduced quality of life so It is important to take protective measures against excessive exposure to heavy metals.  reported that heavy metals might enter the human body through food, water, air, or absorption through the skin when they are exposed to humans in agriculture and in manufacturing, pharmaceutical, industrial, or residential settings. Industrial exposure accounts for a common route of exposure for adults and Ingestion is the most common route of exposure in children.

Animal groups and experimental design
Ninety adult males Wistar rats weighting about (160-220 gram each) was allocated for the experiments. They were then divided into 3 groups of 30 rats each. All groups of rats were kept under standard conditions (Temperature, light, humidity). Rats were fed with standard chow and free tap water when they will be out of metabolic cage. Then, rats were fed with food cooked into two types of locally cooking pots. These were Atmonia and Pepsi can pots. They were known as "Cooking Halla". All test animals were used after the time of adaptation under laboratory conditions. Tarig Elrayah. M. Eltayeb et al., Levels of AST And Alt In Wistar Rats Treated With Heavy Metals Released From Atamonia And Pepsi Cans (=Tins=) As Cooking Pots During Feeding

Feeding technique
Rats ware fed for three months on a food made out of millet flour. Millet flour which prepared by addition of millet flour to cool water and kept for two hours for fermentation. Then the fermented food was cooked in the test pots (Atmonia, Pepsi cans) on a calm fire. Each pot received 9 Kgs of fermented homogenous millet dissolved in tap water for serial cooking's. each cooked food from the two cooking pots collected separately in sterilized clean trays and kept until dry . The dried food was milled until became fine and kept under laboratory conditions until use. Every one rat of the two test group (Atmonia, Pepsi can) was fed into 3gms/day of cooked food using feeding sucker plastic bottle. The control rats ware fed into normal feeding chow as described above. All test and control rats ware kept under laboratory conditions.
All rats ware fed into food consists of protein (meat) to avoid cannibalism.

Collection of Blood Samples
Blood samples were collected by cardiac puncture and allowed to clot for 2 hours at room temperature, followed by centrifugation at 3500 rpm for 10 minutes to obtain the serum.
Estimation of AST and ALT according to Murray et al, 1984 andFischbach et al, 2009 respectively

RESULTS AND DISCUSSION
The results are expressed as Mean (n = 10) per treatment and respective control groups. Levels of significance values was, **p<0.01, considered to be statistically significant.

CV% = 9% SE+ = 1.71
The results are expressed as Mean (n = 10) per treatment and respective control groups. Levels of significance values was, **p<0.01, considered to be statistically significant.

CV% = 15.6% SE+ = 3.44
The results are expressed as Mean (n = 10) per treatment and respective control groups. Levels of significance values was, **p<0.01, considered to be statistically significant. The results are expressed as Mean (n = 10) per treatment and respective control groups. Levels of significance values was, **p<0.01, considered to be statistically significant.

CV% = 15.8% SE+ = 13.6
The results are expressed as Mean (n = 10) per treatment and respective control groups. Levels of significance values was, **p<0.01, considered to be statistically significant. CV%=13.5% SE + =14.2         who reported that heavy element such as Pb Al were considered as the main reasons for hi AST and ALT. The results obtained from this study were supported the assumption that heavy elements released in feeds cooked in cooking pots made of Atmonia and Pepsi cans were the main reasons of high elevation of liver functioning enzymes of Wistar rats such as AST, ALT and ALP. Alkaline phosphatase (ALP) an enzyme also found in the liver, bile ducts, and bones (Ki-Soo Kang, 2013). High levels of these enzymes may cause liver damage or disease, a blocked bile duct, or bone disease. Also agree with (Robert, 2010 reported that AST has cytosolic and mitochondrial forms and is present in tissues of the liver, heart, skeletal muscl brain, pancreas, and lungs, and in white and red blood cells. AST is less commonly referred to as serum glutamic oxaloacetic transaminase and ALT as serum glutamic pyruvic transaminase.  International Journal of Recent Scientific Research Vol. 8, Issue, 5, pp. 16833-16835, May, 2017 and Fig 1 indicated that AST for Atmonia and Pepsi in the first, second and third month compared with the control. These results were clearly in line with Kim, d that heavy element such as Pb, Fe, Cu and ed as the main reasons for high elevation of . The results obtained from this study were supported the assumption that heavy elements released in feeds cooked in cooking pots made of Atmonia and Pepsi cans were of liver functioning enzymes of Wistar rats such as AST, ALT and ALP. Alkaline phosphatase (ALP) an enzyme also found in the liver, bile . High levels of these enzymes may cause liver damage or disease, a blocked bile Robert, 2010) who reported that AST has cytosolic and mitochondrial forms and is present in tissues of the liver, heart, skeletal muscle, kidneys, brain, pancreas, and lungs, and in white and red blood cells. AST is less commonly referred to as serum glutamic oxaloacetic transaminase and ALT as serum glutamic pyruvic  . In analysis of variance, there was significant difference between groups at   ) reported that ALT and AST specific marker of hepatocellular necrosis.
Fig 2 indicate increase level of AST and ALT in the second and third months respectively compared to their controls. In analysis of variance, there was significant difference between groups at P≤0.01.