Agrobacterium mediated gene transformation method is considered as an effective method for transferring foreign genes into plants. Hence, this study was conducted to enhance foreign gene transformation efficiency of cultivars of tomato (Solanum lycopersicum) using Single colony Agrobacterium culture from freshly grown culture of LBA4404 containing T-rep gene construct in pCAMBIA 2301 was grown in 25 ml of Luria broth medium with appropriate antibiotics at 280 C and 180 rpm for overnight. Infection was carried out in antibiotic free MS liquid medium and incubated for co-cultivation in dark for two days. Before the infection overnight grown Agrobacterium culture was re-suspended in antibiotic free Luria broth medium and allowed to grow to the log phase. Explants were then transferred to selection medium (MS salts and B5 vitamins, 3% Sucrose, 1mg/l Zeatin, 0.1mg/l IAA, 80mg/l kanamycin, 300mg/l cephotaxime pH 5.8). After 28 days of incubation at 25+2o C in 16 hr photoperiod condition, explants responded with callus induction were transferred to fresh selection medium. The response of the callus induction on kanamycin selection was more or less similar in all sets of experiments. Most of the survived calluses on second selection medium were observed with shoot induction.
