Present investigation deals with the effect of different nutrients like Sucrose, Boric acid (H3BO3) and salts like Calcium nitrate [Ca(NO3)2], Magnesium sulphate (MgSO4) and Potassium nitrate (KNO3) on in vitro pollen germination of Borassus flabellifer L.(Arecaceae), a plant with immense economic importance. It flowers during March to May which open in early morning (05:30hrs - 06:30hrs).Considerable pollen germination (65%) and tube development (990µm) occurred in 8% Sucrose solution while 100 ppm Boric acid showed 55% germination along with 394µm long pollen tube. However, maximum germinating pollen (80%) along with pollen tube development (1350µm) was observed in 8% Sucrose solution supplemented with 100 ppm Boric acid. Among the salts, maximum 50% pollen germination along with 270µm long pollen tube developed in 200 ppm Magnesium sulphate solution following 41% pollen germination along with 198 µm pollen tube in 200 ppm Calcium nitrate and 32% pollen germination along with 126 µm long pollen tube in 100 ppm Potassium nitrate solution.