Introduction: HIV infected patients are susceptible to a variety of common and opportunistic infections due to progressive decline in their immunity status. Cryptosporidiosis that cause intestinal infections in immuno-compromised patients and most frequently encountered. Due to higher incidence of HIV/AIDS patients in our area, i.e., Raichur district of Karnataka state, it is important to study the opportunistic parasitic diseases like cryptosporidiosis and its correlation with the immune status of the patient, which is one of the predominant infection and can cause severe morbidity and mortality. Detection of this parasite will help in proper management of the patients.
Objectives
· To determine the Cryptosporidiosis in various age groups of HIV/AIDS sero positive patients.
· To determine and compare the Cryptosporidiosis in HIV/AIDS sero-positive patients with diarrhoea and without diarrhoea.
· To detect cryptosporidiosis by ELISA.
· Correlate the CD4 count with incidence of cryptosporidiosis.
Materials and methods
· Stool samples of 110 patients with diarrhoea and without diarrhoea with HIV/AIDS collected during 2014 to 2016 RIMS, Raichur .
· ELISA: Antigen detection technique was employed for cryptosporidial antigen in the faeces for the diagnosis. (DRG Kit ELISA, Germany)
Results: Out of 110 HIV sero-positive 62 patients with diarrhoea and 48 were without diarrhoea. CD4 counts among cryptosporidium parvum isolated HIV/AIDS patients, 78 patients below 200cells/cumm and 27 patients above 200cells/cum. Among 110 HIV/AIDS patients 105 patients were detected Cryptosporidium parvum antigen by ELISA Method. In 110 cases Of HIV/AIDS patients studied, the males were 61 cases and females were 49 cases Among 110 HIV patients, 105 patients were detected Cryptosporidium parvum antigen by ELISA Method
Conclusion: Routine examination for cryptosporidium oocysts in stool should be performed in all AIDS patients.
