Pure CuO nanoparticles and Hexa Methylene Tetra Amine (HMTA) used as a structure directing template added Cuo nanocrystal with diverse weights (0.1, 0.2, 0.3, 0.4, 0.5 gm) were anatomized for structural and morphological research using X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Field Emission Scanning Electron Microscopy (FE-SEM) Transmission Electron Microscope (TEM). The XRD analysis manifested monoclinic cry stallinity in pure and HMTA-added CuO nanocrystals, with an average crystallite size of 21.63 nm and 12-17 nm respectively. The FE-SEM analysis incarnated their nanoflower conformation. The FT-IR spectrum affirmed the presence of Cu-O bonds. The change of structure from nanoflower to nanorods was confirmed by TEM. The antibacterial properties of the as-prepared nanostructures investigated for various human pathogens using disc diffusion method. The result showed the remarkable antibacterial activity both gram positive and gram negative bacteria.
