A series of some new 2,6-bi's (4-Chlorobenzoyl)-3,5-bi's (substituted aryl) tetra hydro 1,4-thiazine -1,1- ioxide has been synthesized by condensing respective aldehydes with 4,4’-dichloro diphenacyl sulphone. They were characterized by 1HNMR, 13CNMR, FT-IR. All the compounds were screened for their in vitro antibacterial activity against Gram positive (Staphylococcus, Bacillus Cereus, B.subtilis) and gram negative organism (Pseudomonas, E.Coli) by disc diffusion method. Among the tested compounds showed the most potent antibacterial activities. The newly synthesized compound were docked with Plasmodium falciparum dihydrofolate reductase-thymidylate synthase (1J3K) using Discovery studio 4.0, the best Lib dock score and Binding energy were obtained for 3,5-Bis(m-nitro phenyl)-2,6-bi’s(p: hlorobenzoyl) tetra hydro 1,4-thiazine 1,1-dioxide (A).
