Utilization of agricultural residues through Biotechnology is becoming more and more significant with the dual goal of waste disposal and value addition. The present research was undertaken to compare the influence of different substrates on tannase activity by Aspergillus flavus by utilizing agro wastes such as tea, coffee, tamarind and white popinac leaves in a submerged fermentation system. To achieve this goal, an in vitro investigation was carried out to assess the enzyme production by the efficient tannolytic fungus Aspergillus flavus isolated from tealeaf waste disposal soil. Among the number of mycoflora Aspergillus flavus showed maximum hydrolyzing zone for tannolytic activity, it was selected as a candidate for enzyme study. Among the different substrates of 0.5g tannase activity by A. flavus was found to be significantly enhanced to a maximum level of 8.96from7.14Umg-1enzyme protein in tealeave satan intracellular level, upto 7th day of incubation and the least tannase activity of was observed in tamarind leaves.
