In past, circumscription at familial and intra-familial levels of Acanthaceae has long been debated. The evidence obtained from any single discipline appears generally to have contradicted evidence from other disciplines. Use of single character is sometimes taxonomically not safer. Placement of the genus Barleria Linn. is one such case. Present authors made an all-pervasive examination of evidence available, which are not used for any taxonomic and phylogenetic assessment earlier, for the genus besides their own anatomical evidence in the acanthaceous plexus. The sum total of evidence warrants that the genus Barleria Linn. is better at home under the subtribe Barlerieae in the tribe Justicieae of the Acanthaceae proper.