Heavy metals are widely distributed in soils, plants and animals in most of their tissues. Heavy metals are included in the main category of environmental pollutants as they can remain in the environment for long periods; their accumulation is potentially hazardous to plants and animals may adversely affect soil productivity. Wheat was grown with Arsenic (As) stress which affects various metabolic activities of wheat at different concentrations. Effect of Arsenic was observed on the pigment content and the result showed that the maximum toxicity was observed at As 150 mg/kg. The reduction of chlorophyll a and b contents of leaves was detected with enhanced Arsenic accumulation in leaves. The protein content was lower in wheat at higher concentration of arsenic that is more toxic to wheat. We studied the toxicity of arsenic on total chlorophyll content and total protein in two varieties (WH-711, C-306) of wheat. Arsenic enters the plant system, may enter the food chain and cause harm to humans and animals. By observing these different parameters of the two varieties of wheat we concluded that the variety WH-711 is more sensitive to heavy metals than C-306.