Objective: The primary goal was to create a novel medium from human urine and compare its Colony morphology and nutritional value with that of nutrient agar medium.
Materials and methods: This study was conducted in the Department of MLT, Government Medical College Thiruvananthapuram, over a period of 3 months. Prior to media preparation, human urine was collected and sterilized. Compare the colony morphology of different bacteria on urine agar medium with nutrient agar and also evaluate the nutritional value of urine agar medium. Result: The novel urine agar medium supports the growth of both gram-positive and gram-negative pathogens. Colonies are well differentiated. This medium enhances the pigment production of Pseudomonas and Staphylococcus. Medium is comparable to a 1/4th concentration of nutrients when compared to nutrient agar.
