The present study was aimed to produce rapid invitro callogenesis in Aristolochia bracteolata Lam. The protocol has been standardized using leaf explant. Aristolochia bracteolata belonging to the family Aristolochiaceae is a multipotent medicinal herb used by traditional healers to treat several diseases as Anti-inflammatory, antipyretic, antimicrobial, antidote and antihelminthic. In order to formulate the micropropagation strategies of this medicinal plants, with various parts of the plant has been used for callogenesis and rapid proliferation was obtained from leaf explants on Murashige and Skoog (MS) medium supplemented with 2-4-D (0.5 mg/l to 1.0 mg/l). The callus possessed a very high regenerative potential was referred to as ‘stock callus’. The extract of leaf callus of Aristolochia bracteolata Lam revealed the presence of tannins, flavonoids, glycosides, terpenoids, saponins and steroids.