The main objective of study was to know the effect of Growth Hormone (GH), Insulin like Growth Factor-I (IGF-I), Epidermal Growth Factor (EGF), Thyroxine (T4) and Follicle Stimulating Hormone (FSH) individually or in combination on in vitro development of goat preantral follicles (PFs). Bicarbonate buffered tissue culture medium 199 (TCM 199B) without any supplementation was used as control medium. Goat PFs (diameter ranging from 150-400µ) were cultured for a period of six days in different concentrations of above mentioned hormones and growth factors individually and also in different combinations. The proportion of PFs exhibiting growth, increase in diameter, antrum formation and extrusion of oocyte in culture were highest when the culture medium was supplemented with GH (2 mIU/ml), IGF-I (20 ng/ml), EGF (50 ng/ml), Thyroxine (1 µg/ml) and FSH (2 ng/ml) individually. The proportion of PFs exhibiting growth, increase in diameter, antrum formation and extrusion of oocytes was 98.07±1.92, 52.27±3.01, 88.46±4.57 and 25.0±0.00 respectively when cultured in T4+FSH+GH+EGF. In conclusion, it can be said that, the addition of GH, IGF-I, EGF, T4 and FSH in different concentrations significantly improved the in vitro development of goat PFs
