Propiconazole, a triazole group of fungicide, broadly used against powdery mildew, rust, leaf spot of cereals and coffee. In the present study, in vitro toxicity of propiconazole was examined with the hepatocellular carcinoma (Hep G2) and mouse embryonic fibroblast (NIH 3T3) cell lines. The viability assays of the cell lines were examined with exposure to the propiconazole in different concentrations (20 to 100µg/ml) and IC 50 values are determined. The results indicate the evidence of propiconazole toxicity, the compound found lethal to Hep G2 cell line, which resulted in 98.66% of cell death and IC 50 values were found to be 41.025 µg/ml. In contrast, NIH 3T3 cells remain viable even in the higher concentration (100 µg/ml). The results would draw attention on propiconazole which found cytotoxic to human cancer cell line and exhibits an anticancer properties. These results suggest that it is necessary to take precaution while using propiconazole.
