Krshnadi Churna is a classical ayurvedic formulation used for the treatment of infantile diarrhea with fiver, asthma in children and infantile vomiting etc. This formulation have Piper Longum (Krshna) (Fr), Aconitum Hetrophyllum (Aruna)(Rt), Cyperus Rotumdus (Musta)(Rz), Pistacia Integerrima (Gall). Aconitum Hetrophyllum is the most important ingredient of the formulation. But some literature reports reveled that few Aconitum species are extremely poisonous in nature. Hence it was necessary to evaluate its toxicity. We have selected Krshnadi Churna for In-Vitro toxicity study. The formula was referred from Ayurvedic Pharmacopoeia of India. The study clearly reveals the nontotoxic activity of formulation against Hela cell line with IC50 value of 40.25 μg/ml for hydroethanolic fraction respectively by MTT assay. Vincristine sulphate was used as standard cytotoxic agent and the IC50 of test samples are compared with standard. The IC50 value of vicristine sulphate is 47.56 μg/ml.
