The natural and synthetic essential oil from the plant Pelargonium graveolens were selected for anti bacterial and antifungal activity on selected bacterial and fungal pathogens. The natural and synthetic oil was preparedseparately with Dimethyl sulfoxide (DMSO)at 25µl, 50µl and 75µl concentration for the sensitivity test. The antimicrobial activity of plant essential oil was tested against clinical isolates. The results showed that natural Pelargonium graveolens oil showed very high effective activity than the synthetic oil at all the three different concentrations. GC/MSanalysis was done to know the chemical comparison of Pelargonium graveolens natural and synthetic essential oils. The chemical compound geraniol present in Pelargonium graveolens natural oil may be showed high activity against the bacterial and fungal isolates.
